Phospho eif2 alpha antibody
WebFeb 26, 2024 · Anti-EIF2S1 (phospho S51) (eIF2-alpha) antibody. Catalog Number: ab32157. Info:Supplier Page View Product Specs View Product. Browse Similar Products: EIF2S1 Western Blot Antibodies. This antibody … WebThis clone failed to immunoprecipitate EGFR Phospho (Tyr845) from whole cell extracts prepared from EGF-stimulated A-431 cells. This recombinant antibody clone is derived from clone A18028A. This clone is predicted to recognize the region corresponding to EGFR Phospho (Tyr845) in both rat (Tyr846) and mouse (Tyr847) orthologs due to complete ...
Phospho eif2 alpha antibody
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WebGuarantees. Rabbit polyclonal antibody to eIF2 alpha (phospho Ser51). This antibody detects endogenous levels of eIF2 alpha only when phosphorylated at Ser51. Synthetic … WebWestern blot - Phospho-eIF2α-S51 Rabbit pAb (AP0342) Western blot analysis of extracts of various cell lines, using Phospho-eIF2α-S51 antibody (AP0342) at 1:1000 dilution.HeLa and C6 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014 ...
WebEIF2AK1, which is activated by heme deficiency and other stimuli, is a major kinase that phosphorylates EIF2-alpha. Phosphorylation of the alpha subunit of EIF2 downregulates protein synthesis in response to various stress conditions. EIF2AK1 has some isoforms with 630aa, 577aa and 422aa. Protocols Publications All (7) KD/KO (1) WB (7) IP (2) WebWestern Blot. Detection of Human Phospho-eIF2 alpha (S52) by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and HEK293T human embryonic kidney cell line untreated (-) or treated (+) with 100nM Calyculin A (Catalog # 1336) and 20 ng/mL Recombinant Human TNF-a (Catalog # 210-TA) for 10 minutes or 20 …
WebJul 16, 2024 · eIF2α Antibody (D-3) is an IgG 1 κ mouse monoclonal eIF2 alpha antibody (also designated EIF2A antibody, eIF2 alpha antibody, or EIF2S1 antibody) suitable for the … WebPhosphorylation of eIF2 alpha blocks the GDP-GTP exchange activity of eIF2 beta, resulting in the suppression of protein synthesis. The phosphorylation of eIF2 alpha is an important …
WebSpecificity. This antibody reacts to human eIF2 alpha/EIF2S1 (eukaryotic translation initiation factor 2 subunit alpha) only when phosphorylated at Ser51. There is no cross-reactivity to eIF2 alpha/EIF2S1 without phosphorylation at Ser51. This antibody may also react to mouse or rat Phospho-eIF2 alpha/EIF2S1 (Ser51). Isotype.
WebSubunit Structure: Heterotrimer composed of an alpha, a beta and a gamma chain. Component of an EIF2 complex at least composed of CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5. … flow current dateWebThe antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns. Storage: … flow curves and calculations document tescomWebPhospho-EIF2S1 (Ser51) antibody for WB, ELISA and reacts with Human, mouse, rat. ... eIF 2 alpha, EIF 2A, EIF 2alpha, EIF2, EIF2A, EIF2S1, EIF2S1 (Ser51), EIF2S1 S51, EIF2S1 Ser51, Phospho-EIF2S1 (Ser51), S51, Ser51 ... EIF2S1 is one subunit of the translation initiation factor EIF2, which catalyzes the first regulated step of protein synthesis ... greek god presentation templateWebMay 11, 2024 · Human Phospho-eIF2 alpha (S52) Antibody Summary. Detects human Phospho-eIF2 alpha (S52) in ELISAs and Western blots. Lyophilized from a 0.2 μm … flow curve rheologyWebJun 8, 2024 · The phosphorylation of eIF2α occurs on serine 51 in response to the activation of one out of four eIF2α kinases 4, 5, 6. EIF2AK1, commonly known as heme-regulated … greek god pushing rockWebNov 26, 2024 · Phospho-eIF2α has increased affinity to the eIF2β subunit and prevents the regeneration of guanosine triphosphate (GTP) in the ternary complex eIF2-GTP-Met tRNAi, thus halting the initiation of ... greek god protectionWebEIF2a (Eukaryotic translation initiation factor 2A) is a heterotrimer composed of three subunits (alpha, beta, and gamma). This translation initiation factor drives binding of … greek god playing cards