WebThis study has expanded the vector toolkit EasyClone-MarkerFree with eight additional intergenic integration sites located on different chromosomes, enabling markerless integration into baker's yeast Saccharomyces cerevisiae … WebUSER & EasyClone plasmids B12407 ARO3K222L + ARO4K229L + pCfB2899 This article. S8 B12408 Ec.AROB + Ec.AROD + pCfB3034 This article ... EasyClone-MarkerFree Integrative Vector B9200 X-2 integration plasmid pCfB2899 Jessop-Fabre et al., 2016 B9201 X-3 integration plasmid pCfB3034 Jessop-Fabre et al., 2016
EasyClone-MarkerFree: A vector toolkit for marker-less …
WebEasyClone-MarkerFree: A vector toolkit for marker-less integration of genes into Saccharomyces cerevisiae via CRISPR-Cas9. Jessop-Fabre MM, Jakociunas T, … WebMay 1, 2024 · A CRISPR-Cas9-based tool for targeted, markerless gene integration into the Y. lipolytica genome and the capability to integrate multiple genes without the need for marker recovery and into sites with known expression levels will enable more rapid and reliable pathway engineering in the yeast. 137 PDF how many calories in flaming hot cheetos bag
Synthetic dynamic growth regulation using ClpXP proteasome …
WebJan 26, 2024 · The Cas9 plasmid pCfB2312 was kindly provided by Dr. Irina Borodina (Technical University of Denmark, Denmark) as a part of the “EasyClone-MarkerFree” vector toolkit [ 14 ]. The sgRNA plasmids pCfB9336, pCfB9341, and pCfB9344 were constructed previously [ 15 ]. WebDec 31, 2024 · TL;DR: The EasyClone-MarkerFree vector toolkit as discussed by the authors enables the integration of linearized expression cassettes into defined genomic loci, facilitated by CRISPR/Cas9, which is recruited to the chromosomal location by specific guide RNAs expressed from a set of gRNA helper vectors. WebApr 24, 2024 · The EasyClone-MarkerFree toolkit allows the Cas9-mediated integration of up to three marker-less cassettes simultaneously 23 and was recently expanded to … high rise employment