Dicarboxypropylation
WebOct 24, 2012 · These NLRP3 modifications include SUMOylation [163][164][165], nitrosylation [166][167] [168], alkylation [169,170], acetylation [171], and dicarboxypropylation, which is a rare modification ... WebDec 1, 2024 · Specifically, itaconate inactived irreversibly the thiol groups of proteins in a process called 2,3-dicarboxypropylation [20]. Several targets of 2,3-dicarboxypropylation have been identified, including Kelch-like ECH-associated protein 1(KEAP1), LDHA, and other enzymes in the glycolytic pathway [21].
Dicarboxypropylation
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Webcompetition ratio denoting greater enrichment in 2,3-dicarboxypropylation of that protein by OI. JAK1 and KEAP1 are highlighted as top hits in this screen modified by OI. (e) Uniprot amino acid sequence alignment of rat, human, and mouse JAK1 pseudokinase and kinase domains, showing conservation of the three species. WebItaconate-derived protein dicarboxypropylation provides a robust measure of the cellular capacity to increase immunometabolism, and we observe that this is recognized by an in-house antibody detecting succinate moieties. Using this antibody as a tool, we determined that isolated NDUFS4 KO microglia do not accumulate fumarate or itaconate ...
WebFeb 1, 2024 · This is particularly the case with more electrophilic derivatives, such as 4OI. Examples of dicarboxypropylation by ITA include JAK1 that inhibits M2 macrophage polarization [18] and several glycolytic enzymes, including aldolase and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and the pyroptosis regulator Gasdermin D 17, 19, 20. WebJul 20, 2024 · These NLRP3 modifications include SUMOylation [163,164,165], nitrosylation [166,167,168], alkylation [169,170], acetylation , and dicarboxypropylation, which is a rare modification mediated by 4-octyl itaconate, a derivative of immunomodulatory metabolite itaconate [172,173]. Collectively, phosphorylation and various PTMs are critical ...
WebApr 1, 2024 · Further characterization of the immunometabolic crosstalk can provide insight to chronic infection and persistence of intracellular pathogens. In addition to its role in supplying energy demands ... WebDicarboxylic Acid. Dicarboxylic acids (products of the CYP4A-mediated pathway) play a regulatory role in the hepatic disposition of nonesterified fatty acids by activating the …
WebJun 15, 2024 · The unsaturated dicarboxylic acid structure of itaconate renders it slightly electrophilic and mediates an interaction with thiol functional groups through 2, 3-dicarboxypropylation in the cytosol (Hooftman et al., 2024; Peace and O’Neill, 2024). Itaconate has anti-inflammatory and anti-oxidant properties that have been partly …
WebDec 30, 2024 · In the cytosol, itaconate alkylates KEAP1, a novel PTM termed 2,3-dicarboxypropylation, and GSH to form an itaconate-GSH adduct, or 2,3-dicarboxypropyl-GSH (4). This in turn activates the anti ... graph waves calculatorWebDicarboxylic acid definition, any of the organic compounds that contain two carboxyl groups. See more. chitchat bluetoothWebThese NLRP3 modifications include SUMOylation [163][164] [165], nitrosylation [166][167][168], alkylation [169,170], acetylation [171], and dicarboxypropylation, which is a rare modification ... chit chat birdWebDec 1, 2024 · Itaconate alkylates cysteine residues (i.e., 2,3-dicarboxypropylation or itaconation) on kelch-like ECH-associated protein 1 (KEAP1), allowing NRF2 to accumulate and increase the expression of downstream anti-inflammatory genes (such as heme oxygenase 1 and glutathione) and decrease IL-1β and IL-6 transcription in mouse and … graph wearWebMar 28, 2024 · Itaconate alkylates cysteine residues 151, 257, 288, 273 and 297 on the protein KEAP1, enabling Nrf2 to increase the expression of downstream genes with anti-oxidant and anti-inflammatory ... chitchat buckleWebSupporting: 2, Mentioning: 45 - Itaconate is an anti-inflammatory metabolite involved in pathogen–macrophage interactions, but the mechanisms underlying its effect are not fully understood. Competitive cysteine profiling has been performed to interrogate itaconate’s reactivity in cell lysates, but methods for analyzing targets of itaconation directly in living … chitchat bluetooth speakerphoneWebC816/817 and C715 are located in the pseudokinase domain of JAK1, while C943 and C1130 are within the kinase domain, and with 3D modeling, we speculate that the 2,3 … chit chat blog